Silver enhancement of polymerised diaminobenzidine: increased sensitivity for immunoperoxidase staining.

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Silver enhancement-of polymerised diaminobenzidine: Increased sensitivity for immunoperoxidase staining

Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for K and A light immunoglobulin chains and CD3 antige...

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Silver enhancement of polymerised diaminobenzidine: increased sensitivity for immunoperoxidase staining.

Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for kappa and lambda light immunoglobulin chains and C...

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Silver nucleation on gold has been exploited for signal amplification and has found application in several qualitative and quantitative bio-sensing techniques, thanks to the simplicity of the method and the high sensitivity achieved. Very recently, this technique has been tentatively applied to improve the performance of gold-based immunoassays. In this work, the exploitation of the signal ampl...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1991

ISSN: 0021-9746

DOI: 10.1136/jcp.44.9.756